Mitokyne: A Ratiometric Raman Probe for Mitochondrial pH

Mitochondrial pH (pHmito) is intimately related to mitochondrial function, and aberrant values for pHmito are linked several disease states. We report the design, synthesis, application of mitokyne 1—the first small molecule sensor stimulated Raman scattering (SRS) microscopy. This ratiometric probe can determine subtle changes in response external stimuli inhibition both electron transport chain ATP synthase with inhibitors. In addition, 1 was also used monitor dynamics a time-resolved manner subcellular spatial resolution during mitophagy providing powerful tool dissecting molecular cell biology this critical organelle.